FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429570
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1784350.7615803448377413TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG230850.060.77672246
CGTATGC231800.060.7401744
ATGCCGT242250.057.8730547
TCGTATG248250.056.83013543
GTATGCC249450.056.3718345
TGCCGTC251250.055.53533648
GCCGTCT262650.052.93846549
CTCGTAT268200.050.3344742
ACTGACC325400.043.72533832
AATCTCG308900.043.01914239
ATCTCGT312000.042.63440340
TCTCGTA316850.042.27738641
CACTGAC348600.041.0940831
CCGTCTT342800.040.96920850
AAAAGGG260350.039.29149670
CTGCTTG364700.037.9712557
GACCAAT374250.037.04498735
TGAAAAA396700.036.01132262
TCACTGA403750.035.5490130
GTCACTG408650.035.38710829