Basic Statistics
Measure | Value |
---|---|
Filename | HFWHCBGXY_n01_shoot02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23429570 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 178435 | 0.7615803448377413 | TruSeq Adapter, Index 4 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 23085 | 0.0 | 60.776722 | 46 |
CGTATGC | 23180 | 0.0 | 60.74017 | 44 |
ATGCCGT | 24225 | 0.0 | 57.87305 | 47 |
TCGTATG | 24825 | 0.0 | 56.830135 | 43 |
GTATGCC | 24945 | 0.0 | 56.37183 | 45 |
TGCCGTC | 25125 | 0.0 | 55.535336 | 48 |
GCCGTCT | 26265 | 0.0 | 52.938465 | 49 |
CTCGTAT | 26820 | 0.0 | 50.33447 | 42 |
ACTGACC | 32540 | 0.0 | 43.725338 | 32 |
AATCTCG | 30890 | 0.0 | 43.019142 | 39 |
ATCTCGT | 31200 | 0.0 | 42.634403 | 40 |
TCTCGTA | 31685 | 0.0 | 42.277386 | 41 |
CACTGAC | 34860 | 0.0 | 41.09408 | 31 |
CCGTCTT | 34280 | 0.0 | 40.969208 | 50 |
AAAAGGG | 26035 | 0.0 | 39.291496 | 70 |
CTGCTTG | 36470 | 0.0 | 37.97125 | 57 |
GACCAAT | 37425 | 0.0 | 37.044987 | 35 |
TGAAAAA | 39670 | 0.0 | 36.011322 | 62 |
TCACTGA | 40375 | 0.0 | 35.54901 | 30 |
GTCACTG | 40865 | 0.0 | 35.387108 | 29 |