FastQCFastQC Report
Wed 10 Aug 2016
HFWHCBGXY_n01_shoot01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWHCBGXY_n01_shoot01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26775441
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC6059602.263118654142802TruSeq Adapter, Index 2 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG723950.066.0314446
CGTATGC724300.065.8942244
ATGCCGT719850.065.0896247
GTATGCC754250.064.1356145
TCGTATG727800.064.00136643
TGCCGTC720000.063.85600348
TATCTCG755550.062.8077839
GCCGTCT719800.062.4637349
CTCGTAT763500.060.9464142
GTATCTC810000.059.0159838
CCGATGT946200.057.96738433
GATGTAT941450.057.9030835
CGATGTA948250.057.8645434
TGTATCT849450.057.6366837
ATGTATC928400.057.6193336
ATCTCGT818050.057.50037840
TCTCGTA816450.056.8635641
AAAAGGG679200.056.71595470
ACCGATG981500.056.02797332
CCGTCTT824700.055.42217650
Produced by FastQC (version 0.11.2)