FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5642682
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85800.15205535240157073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13300.030.8979211
CACACGT14700.028.26303912
ACGTCTG14750.027.57947515
GATCGGA15550.027.1880041
CACGTCT14950.026.9098514
AGCACAC15700.026.60376510
ACACGTC15550.026.59419413
CGTCTGA16250.024.62202816
GAGCACA18550.023.1216759
GAACTCC17200.022.89216621
CAGTCAC15200.022.83795427
AGAGCAC18950.022.6336178
CCAGTCA15800.022.25562926
TCCAGTC16900.021.73933225
TCGGAAG21700.019.8819983
CTCCAGT19700.019.67500324
ACTCCAG20100.019.16453223
GTCTGAA22750.018.08936917
AGTCACG10250.018.0358128
CTGAACT22850.018.02091619
Produced by FastQC (version 0.11.5)