FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS841-15h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS841-15h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14063127
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG32750.031.53153629
CACGGCT32950.031.13530731
TCACGGC33500.030.55697330
GCACACG34150.030.1071211
AGTCACG34950.029.48234628
CGGCTAC35850.028.3656733
ACACGTC37050.028.17562113
CACACGT37550.027.92027912
ACGGCTA37150.027.73642232
CACGTCT38650.026.89281314
ACGTCTG38550.026.84585215
GATCGGA38500.026.7938521
GGCTACA40250.025.15303234
AGCACAC41700.025.08786610
CGTCTGA42000.024.85492116
GAGCACA43000.024.2770779
CAGTCAC44150.023.74646427
CATCTCG32000.023.0604439
GAACTCC44650.022.87589621
TCCAGTC45850.022.66973325
Produced by FastQC (version 0.11.5)