FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS841-15h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS841-15h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14042555
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27150.025.0257411
ACACGTC28950.023.46973613
CACACGT29350.023.2265312
ACGTCTG29700.023.10432215
CACGTCT31300.022.06703214
GATCGGA30700.022.0078051
CGTCTGA32150.021.4136316
CAGTCAC34600.019.44217927
AGCACAC35300.019.31157710
TCCAGTC36600.018.50270725
GTCACAT37700.018.1418729
GAGCACA38650.017.7541649
AGTCACA37750.017.70065528
GAACTCC39350.017.66703221
CCAGTCA38800.017.6275426
AGAGCAC41000.016.7365448
ACTCCAG44250.015.81236923
GTCTGAA44050.015.52664217
TCGGAAG46200.015.0546453
TCTGAAC48600.014.53594318
Produced by FastQC (version 0.11.5)