FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS841-0h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS841-0h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17035603
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG48950.033.7357611
TATCGGG42250.031.31105839
TCGGGGG24050.030.87066541
ACACGTC54400.030.31462513
CACACGT54800.030.09335112
GATCGGA55300.029.8947561
ATCGGGG40450.029.75653840
ACGTCTG56200.029.46378915
CGGGGGT18400.028.85634442
CGTCTGA57650.028.839816
CACGTCT58500.028.26692614
CACTAGC61050.027.41791231
AGCACAC61550.027.01257910
CAGTCAC62450.026.65915527
GAGCACA63500.026.5373639
GAACTCC64900.025.54876521
CCAGTCA65700.025.51161826
TCCAGTC65450.025.40281925
AGAGCAC67950.024.7663388
AGTCACT69050.024.3064928
Produced by FastQC (version 0.11.5)