FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS841-0h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS841-0h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10998905
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG374950.3408975711673117TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52250.037.9780711
CACACGT54800.036.33400712
GTCACAC54700.036.2359129
ACACGTC55300.036.0054913
CACGTCT55500.035.7135914
ACGTCTG56100.035.33162715
GATCGGA55750.035.225791
AGCACAC58900.033.84316610
CGTCTGA58800.033.5944716
TATCTCG57850.033.17387841
CAGTCAC60050.032.85769727
TCCAGTC61150.032.2666425
AGTCACA62050.031.90740428
GAGCACA63250.031.8001799
CACACTG62500.031.60567531
GAACTCC62200.031.57726121
CCAGTCA63600.031.02366326
CTCGTAT60200.030.90719644
AGAGCAC65850.030.7154188
ATCTCGT64050.030.10317242
Produced by FastQC (version 0.11.5)