FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS840-15h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS840-15h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16465249
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23900.022.12168911
AGTCACG25400.020.99244328
GTCACGA26050.020.4686429
CACACGT27200.019.93409312
CGATCAG26850.019.52360333
ACACGTC27650.019.1214613
CACGATC28750.018.23334931
GATCGGA28850.018.1895351
CACGTCT30500.017.55599614
ACGTCTG31250.017.06265815
GATCTCG28600.016.9916739
AGCACAC33150.016.49191510
GTATGCC31750.015.305881545
CAGTCAC35950.015.27000827
CGTCTGA34900.015.21370316
GAGCACA36700.014.8966479
TCCAGTC36550.014.58845625
CGTATGC35400.014.363276544
GAACTCC37750.014.18431421
CCAGTCA39550.013.65252926
Produced by FastQC (version 0.11.5)