FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS840-15h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS840-15h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11472827
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG206700.18016483644353742TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT47250.036.37769331
GCACACG48750.035.58142511
CGAGTGG48150.035.51083433
GTCACGA49050.034.7675229
GATCGGA49650.034.478121
ACGAGTG49950.034.36628732
AGTCACG50450.033.89190728
CACACGT52100.033.50946812
ACACGTC52100.033.46628613
TCACGAG51450.033.18944530
ACGTCTG52600.033.10539615
CACGTCT53750.032.22966814
CGTCTGA54450.031.93928316
AGCACAC56250.030.9972210
GAACTCC56150.030.85208521
AGAGCAC56700.030.5924938
CAGTCAC56350.030.30340627
TCCAGTC57250.030.25929325
GAGCACA58650.029.7287929
CCAGTCA59200.029.07256126
Produced by FastQC (version 0.11.5)