FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS840-0h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS840-0h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13925729
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28500.027.7867111
ATCGGGG24100.025.95177740
CGGGGGT11200.025.91260342
GTCACAC30800.025.78477529
TCGGGGG16500.025.77016441
ACACGTC31400.025.50701713
GATCGGA32000.024.707041
CACACGT32500.024.5744812
ACGTCTG33350.023.94814315
CACGTCT33750.023.86429414
CAGTCAC36550.022.46698627
CGTCTGA35700.022.37172516
AATCGGG29750.021.93059339
AGCACAC37300.021.71373610
GAGCACA38050.021.285749
CCAGTCA39800.020.74542626
TCCAGTC40000.020.64169725
GAATCGG31850.020.62589538
GAACTCC40900.019.91244721
AGTCACA41050.019.89448728
Produced by FastQC (version 0.11.5)