FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS840-0h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS840-0h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922529
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG48100.035.54808433
GCACACG49300.035.047911
GATCGGA51950.032.903421
ACGTCTG53250.032.70160715
ACACGTC53450.032.53714813
CACACGT54150.032.1165412
CGTACGT53450.031.9478634
CGTCTGA54550.031.79855216
CACGTCT55550.031.14511714
ACCGTAC56600.030.28909132
GAACTCC57750.029.88072221
AGCACAC58650.029.53727310
GTCACCG59000.029.09512529
CAGTCAC60050.028.7362527
GTACGTA60450.028.5088835
TCCAGTC60700.028.4285325
GAGCACA61850.028.0818239
AGAGCAC62350.027.964888
CCAGTCA61900.027.8047226
ACTCCAG62900.027.50574523
Produced by FastQC (version 0.11.5)