FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS421-15h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS421-15h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12992093
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12400.011.43057711
ACACGTC15250.010.0320213
ACTTAGG15100.09.6846932
CTTAGGC14550.09.43226833
ACGTCTG15750.08.7136215
GATCGGA14800.08.6736241
CACGTCT17250.08.60803714
CACACGT16750.08.59635712
TCCAGTC21600.07.49942725
CGTCTGA19150.07.40152216
GAGCACA20650.06.97283279
CAGTCAC22200.06.89136527
TTAGGCA21050.06.84033234
TAGGCAT21700.06.73911635
GAACTCC23500.06.70161521
AGCACAC21200.06.6858110
CCAGTCA23700.06.550132326
AGGGGGG12604.0017767E-115.7196161
AGAGCAC25050.05.56843668
CACTTAG28000.05.544218531
Produced by FastQC (version 0.11.5)