FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS421-15h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS421-15h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12373799
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21650.028.99321611
AGTCACG23000.026.90016428
TCGGGGG13900.026.22106243
ACACGTC25200.024.73029713
GATCGGA25300.024.5705281
CACACGT26400.024.03229512
ACGTCTG26450.023.81674615
CACGTCT27250.022.95241214
GTCACGT27700.022.4983229
AGCACAC28300.022.2598210
CGTTTCG28450.022.1424633
CGTCTGA29350.021.00354216
CAGTCAC30250.020.75051527
GAGCACA31050.020.288349
TCCAGTC32500.019.59084325
AGAGCAC34100.018.6716258
GTTTCGG33550.018.64242234
CCAGTCA35550.017.72019426
CGGGGGT8450.017.30639844
GAACTCC36750.017.26401721
Produced by FastQC (version 0.11.5)