FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS421-0h-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS421-0h-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16309545
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22250.021.2340211
CACACGT24800.018.59709712
ACACGTC26600.017.59238213
GATCGGA27500.016.5454981
CACGATC25050.015.89675536
ACGTCTG29400.015.84039315
GATCTCG25800.015.60904339
CGTCTGA31050.015.43337516
CGATCTC26300.015.14120638
CACGTCT30500.015.12157314
CAGTCAC32400.014.58200527
AGCACAC33500.014.37182510
TCCAGTC34700.013.93965225
CATCACG34150.013.76887833
GAGCACA36100.013.6483419
CCAGTCA37250.012.92499626
AGTCACA36850.012.88213628
GTATGCC28900.012.45561245
GTCACAT38450.012.28756829
GAACTCC38450.012.17054421
Produced by FastQC (version 0.11.5)