FastQCFastQC Report
Fri 14 Aug 2020
HFWF7AFX2_n01_SS421-0h-GFP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWF7AFX2_n01_SS421-0h-GFP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12372394
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG143160.11570921520927963TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG105000.040.96801411
AGTCACG104350.040.5979628
CACGTGG104750.040.57179631
ACACGTC106950.040.09483313
ACGTGGC105950.040.04857332
CACACGT108100.039.83479312
GATCGGA107150.039.8344271
ACGTCTG107400.039.73830815
GTCACGT107150.039.5370729
CACGTCT108850.039.37430214
TCACGTG109500.038.8323830
AGCACAC111200.038.6433610
CGTCTGA111050.038.57416
GGCCTTA110800.037.74730336
GAGCACA114700.037.62119
CGTGGCC112200.037.5570333
CAGTCAC113200.037.5432527
TCCAGTC115200.036.89145725
AGAGCAC117550.036.8238148
CCAGTCA115850.036.6262126
Produced by FastQC (version 0.11.5)