FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32221513
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC881150.27346636391655477TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT113750.057.26881842
TCGTATG116500.056.36916743
TCTCGTA115500.056.34014541
CGTATGC118100.055.6671944
ATGCCGT121300.054.4578747
ACGGCTA124800.052.91015232
CGGCTAC124550.052.59679433
TATGCCG126300.052.27401746
ATCTCGT127000.051.15491540
TGCCGTC128850.051.10436248
CCGTCTT129050.050.89079350
GCCGTCT132450.049.74323749
CATCTCG135500.048.1266239
CGTCTTC137900.047.6493551
TCACGGC139550.047.46521830
GTCACGG146800.045.09757629
CGTCTGA150850.044.67601816
AGTCACG149800.044.21770528
CACGGCT153600.043.07842331
ACACGTC159850.042.29086313
Produced by FastQC (version 0.11.2)