FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34172197
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1157250.33865250162288363TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT138600.060.260342
TCTCGTA140250.059.72580741
TCGTATG136800.059.36551343
CGTATGC144300.058.46608744
ATGCCGT144200.057.29070747
TATCTCG152700.056.138739
TATGCCG149800.056.0838146
TGCCGTC150850.054.7186448
ATCTCGT155700.054.540640
CCGTCTT155100.053.51336750
GCCGTCT151300.053.33067349
CGTCTTC157400.051.19563351
CGTCTGA190550.050.7025316
ACGTCTG199700.048.4311215
ACACGTC202900.047.7018613
TCGCGTA9900.046.6724841
GCTTATC207450.045.4291436
GTATGCC189350.045.16492545
CACGTCT218450.044.35433614
GATCGGA228450.042.456141
Produced by FastQC (version 0.11.2)