FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33883855
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC7365322.173695997695658TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA847350.066.4866641
ATCGGAA853950.066.148112
TCGGAAG862900.065.107893
CGGAAGA866700.064.612394
TCGTATG844650.064.24190543
CTCGTAT830700.064.21496642
TCTCGTA830650.064.0921241
CGTATGC847800.064.0810544
CGATCAG851550.063.84087433
ATGCCGT852100.063.73580647
ACGATCA857150.063.6834732
CGTCTGA866950.063.621916
GTCACGA857800.063.57406629
ACACGTC872450.063.4968113
TATGCCG857100.063.39239546
ACGTCTG871900.063.35561815
CACGATC862400.063.299731
GATCTCG840200.063.2748339
GCCGTCT858750.063.125849
TGCCGTC860300.063.1043648
Produced by FastQC (version 0.11.2)