FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37968301
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52500.032.47050542
TCGTATG53700.031.61524643
TCTCGTA55650.030.88403541
CGTATGC58050.030.69491444
TATGCCG60900.029.31577946
AATCTCG63350.028.0131839
ATGCCGT62750.027.78208247
ATCTCGT69600.024.99511340
TGCCGTC74850.023.80544348
GCCGTCT78100.022.41188649
CCGTCTT80700.022.21028750
CGTCTGA101850.020.09837516
CGTCTTC90300.019.73234251
ACACGTC110800.018.7270613
ACGTCTG116650.017.96808815
TCGCGTA8000.016.626941
GTATGCC111150.016.56658245
CACGTCT130400.016.18076114
CGCGTAT8450.015.74150642
ATCGGAA143750.014.4590432
Produced by FastQC (version 0.11.2)