FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33625335
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC385940.11477655166855587TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT60450.046.0935642
TCGTATG60750.046.03989843
TCTCGTA60700.045.49997341
CGTATGC64300.043.88140544
ATGCCGT64750.043.79165347
TATGCCG70750.040.32544746
TGCCGTC73100.038.933348
GCCGTCT74750.037.55993349
ATCTCGT77600.036.17681540
CCGTCTT78800.035.8511550
CATCTCG82650.034.0930239
CGTCTTC84400.033.51288251
CGTCTGA100250.029.59915416
ACGTCTG106700.027.90756615
ACACGTC109150.027.18513713
GTATGCC109050.026.48426445
CACGTCT126550.023.75153714
ATCGGAA134950.022.0403122
GATCGGA134500.021.8658521
CACACGT142600.021.0290712
Produced by FastQC (version 0.11.2)