FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22333815
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC263910.11816610820856177TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42400.045.8190642
TCGTATG45650.043.1714343
CGTATGC46650.042.47319844
TCTCGTA46200.041.82290641
TATGCCG48050.041.23485646
ATGCCGT50850.039.23966647
ACGATCT51050.037.84113337
CACGATC53050.036.54612436
ATCTCGT53000.036.5220140
CGATCTC53550.036.144838
GATCTCG54350.035.61412439
ATCACGA56900.034.87240634
TCACGAT56400.034.87139535
TGCCGTC59850.033.51428648
CCGTCTT60250.032.88647550
GCCGTCT61300.032.4943549
CGTCTTC66250.030.2772951
CATCACG67900.029.42847433
GTATGCC77350.026.02302245
CGTCTGA110950.018.00860416
Produced by FastQC (version 0.11.2)