FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32599981
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT129700.014.738611
TATGCCG21650.014.22892248
ACGGCGA35150.012.14480414
ACCTTCG84950.011.62316161
TGGGGGG205050.011.5422581
GTCGCAT20950.011.3639351
GTGCATT113200.010.5465841
CTCGTAT85900.010.3117231
GTCGCTT75400.010.2618291
CAATTGA51500.010.2594114
CGGCGAC41900.010.1882415
GTCGTTT74650.010.08352851
TCCGTAT32100.09.92071537
TTCGGAA103200.09.83822764
CGCTAGT10700.09.8161541
CGCGTTA11850.09.74451516
CCGATCG21700.09.6748783
CCGTTTA24350.09.6280092
GTCCTAT45650.09.5867881
TCGTATC65350.09.5844712
Produced by FastQC (version 0.11.2)