FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28733377
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT375610.1307225391571621TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55500.052.0992548
TCGTATG81200.036.2138945
CGTATGC83850.034.693146
AATCTCG90000.032.0876941
ATGCCGT96000.030.70411749
ACACGTC105500.027.82724613
CGTCTGA107350.027.25023516
CTCGTAT108000.026.93636944
TCTCGTA111250.026.58866743
AGTCAAC110600.026.48328434
ACGTCTG115100.025.74949815
TCAACAA115600.025.51966936
TGAAAAA116450.025.22394464
TTGAAAA118350.024.61218363
GTCAACA122400.024.24461235
GTATGCC124350.023.59071547
GCCGTCT124700.023.4963951
CACGTCT129300.023.02992414
CCGTCTT129200.022.51611952
CACACGT138300.021.50581612
Produced by FastQC (version 0.11.2)