FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32822236
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1090570.33226560189257065TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG144050.058.42209646
TCGTATG150350.055.03987543
CGTATGC156450.053.90426344
CTCGTAT170100.048.50383442
GTATGCC183400.046.2122745
ATGCCGT184500.045.31011647
ACGTCTG199050.045.30091515
ACACGTC201550.044.61761513
CGTCTGA202350.044.4247416
TCTCGTA190550.043.2245541
CACGTCT220200.040.9499314
TGCCGTC208450.039.90234848
GCCGTCT207400.039.7174249
CACACGT227550.039.6116612
ATCGGAA244300.036.767082
ATCTCGT231250.036.0553440
GATCGGA249800.036.034941
AGATCAT258800.034.69706735
GCACACG264700.034.2240611
CCGTCTT242050.034.2195450
Produced by FastQC (version 0.11.2)