FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39795429
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5181711.3020867296090715TruSeq Adapter, Index 6 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG743470.18682296401428417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG657160.16513454346729117No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG422450.10615540794898831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG571500.065.0994446
TCGTATG575300.064.8199343
ACGCCAA584900.063.8482432
CGTATGC584250.063.72184444
CGCCAAT598350.062.27992633
GTCACGC601100.062.16472629
GTATGCC605550.061.53248245
CCAATAT606200.061.49167635
TATCTCG603450.061.48541339
CTCGTAT609550.060.8606142
AGTCACG618450.060.59615728
TCTCGTA615800.060.22555541
ACACGTC627600.060.13093613
ACGTCTG628600.059.91280715
CGTCTGA630300.059.7847516
TCACGCC629850.059.43813330
ATGCCGT631950.058.9497447
TGCCGTC639200.058.25395648
GCCAATA640450.058.19236434
CACACGT649650.058.1381912
Produced by FastQC (version 0.11.2)