FastQCFastQC Report
Tue 22 Nov 2016
HFW7KBGXY_n01_ba54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW7KBGXY_n01_ba54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36797961
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1070840.2910052543400435TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG153950.056.59573746
TCGTATG160850.053.6661643
CGTATGC164400.053.10660644
GTATGCC192650.045.50069445
ACACGTC201900.045.21692313
ACGTCTG204350.044.69180715
CTCGTAT184350.042.49464842
ATGCCGT205350.042.46355447
CGTCTGA214100.042.4285116
ATCGGAA227700.040.2472042
CACGTCT227800.040.1529514
AGTGATC198300.040.13110736
TCTCGTA194850.040.07879641
GATCGGA230150.039.6906171
CACACGT230750.039.66957512
TGCCGTC223300.038.90897848
GCCGTCT225850.038.2228249
GCACACG262150.034.98473711
GATCTCG235900.033.29659339
ATCTCGT239100.032.7634940
Produced by FastQC (version 0.11.2)