FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9679904
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6387916.599146024588673No Hit
TGCCGCTATTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC4948595.11223045187225No Hit
TGCCGCTATTTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAAC4861965.022735762668721No Hit
TGCCGCTATTTCTCTGGGTACCGCGTATAGCTTCTCGCTATTATTCCAAC2845992.940101472080715No Hit
TGCCGCTATTTCTCTGGGTACCGTAATCGTGTTCTCGCTATTATTCCAAC1739771.7973008823227998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC162420.16779092024053133No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115850.11968093898451886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTTA1724400.097.755424104
CCGCTAT1953200.091.634213
AAGCAGT398200.090.9342351
GCCGCTA1990000.090.632382
TTTCTCT1950100.090.352839
CTATTTC1970450.089.9395146
GCTATTT1993350.089.3122945
TGCCGCT2015500.089.127961
ATTTCTC1991050.088.686498
AGCAGTG411500.087.5217742
TATTTCT2109200.083.8893057
GCAGTGG469650.076.5385743
CAGTGGT478600.074.841224
GGTATCA478750.074.6685948
GTATCAA481000.074.186389
GCCGCGA17700.073.964822
GTGGTAT490100.073.254836
AGTGGTA491000.073.15615
TGGTATC503000.071.4424747
CCGCTAG14250.066.8488543