FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_2-SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_2-SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18582439
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCTATTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC576433231.02031977610689No Hit
TGCCGCTATTTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAAC472297725.41634604585544No Hit
TGCCGCTATTTCTCTGGGTACCGCGTATAGCTTCTCGCTATTATTCCAAC371597219.99722426103484No Hit
TGCCGCTATTTCTCTGGGTACCGTAATCGTGTTCTCGCTATTATTCCAAC17676939.512707131717208No Hit
TGCCGCTATTTCTCTGGGTACCAACACTTACTTCTCGCTATTATTCCAAC1084420.5835724793715185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1070970.5763344628764825No Hit
TGCCGCTATTTCTCTGGGTACCGAGCGATCTTTCTCGCTATTATTCCAAC910520.489989500301871No Hit
TGCCGCTATTTCTCTGGGTACCTATTTGTGTTTCTCGCTATTATTCCAAC656140.35309681360988193No Hit
CGCCGCTATTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC326600.1757573373441452No Hit
TGCCGCTATTTCTCTGGGTACCCAATGTTGTTTCTCGCTATTATTCCAAC270580.14561059503545257No Hit
CGCCGCTATTTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAAC259720.13976636759039004No Hit
TGCCGCCATTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC230660.12412794682118962No Hit
TGCCGCTATTTCCCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC221870.11939767433112522No Hit
TGCCGCTACTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC220910.11888105754040146No Hit
CGCCGCTATTTCTCTGGGTACCGCGTATAGCTTCTCGCTATTATTCCAAC208750.11233724485790052No Hit
TGCCGCTATTCCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC193490.10412519045535412No Hit
TGCCGCCATTTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAAC187590.10095014976236436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCCA154.5939087E-4104.141821
TGCCGCT17698600.0104.052971
GACTCTA1100.0104.0389252
GACACTA306.557457E-9104.038922
GCTCCAC154.612006E-4104.038922
CCGCCAC252.6960697E-7104.00283
CTCGTAG154.61838E-4104.002793
ACGTCCA154.622633E-4103.978735
CTAGGTC500.0103.975386
CTATGTC3700.0103.975376
CTATCCC450.0103.975376
CGATGTC403.6379788E-12103.975376
GCCGCCA70700.0103.965342
CCGCTAC65950.0103.923953
TGCCGCC71250.0103.9225851
GCCGCTA17859250.0103.7910462
GCCACTA20100.0103.780122
CCGCTAT17820100.0103.703993
CCGCCAT70650.0103.634773
CGCCGCT101900.0103.630821
Produced by FastQC (version 0.11.5)