FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14618037
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC570170.3900455307371297No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG189630.1297233000573196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168580.11532328177853154No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA162850.11140346682663342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1163050.096.7319641
AGCAGTG1255450.089.443082
GCAGTGG1396400.080.248473
CAGTGGT1416000.079.245714
GGTATCA1429200.078.438518
GTGGTAT1436500.078.061346
TGGTATC1438950.077.935667
AGTGGTA1446850.077.591565
GTATCAA1457950.076.8275459
CAACGCA1457750.041.86655812-13
GCAGAGT1457350.041.82168216-17
AGAGTAC1460200.041.608618-19
ATCAACG1484800.041.16154510-11
TACATGG1483950.040.5117122-23
CATGGGG530000.039.34343324-25
AGTACAT1542750.039.24064320-21
CAGAGTA1466100.038.24941618-19
AACGCAG1473250.038.1778314-15
TCAACGC1476650.038.06853512-13
ACGCAGA1616850.037.8146314-15
Produced by FastQC (version 0.11.5)