FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18054895
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC684800.37928772224928475No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG236510.13099494624587957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207520.11493835882180428No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA197440.10935538534009752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1481500.096.843321
AGCAGTG1606550.089.221872
GCAGTGG1781500.080.2237243
CAGTGGT1814500.078.780024
GGTATCA1832150.078.0550168
GTGGTAT1837850.077.767036
TGGTATC1845300.077.480167
AGTGGTA1851300.077.247595
GTATCAA1874050.076.2102059
CAACGCA1872000.041.8667212-13
GCAGAGT1868450.041.80226516-17
AGAGTAC1868650.041.65878718-19
ATCAACG1912800.041.01039510-11
TACATGG1900150.040.5452122-23
CATGGGG686000.039.57781624-25
AGTACAT1963600.039.47624220-21
CAGAGTA1878550.037.98793418-19
AACGCAG1890300.037.9328114-15
ACGCAGA2069250.037.92500714-15
TCAACGC1900000.037.7375812-13
Produced by FastQC (version 0.11.5)