FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12774570
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC484160.3790029723113968No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG169500.1326854837383959No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154440.12089643721863044No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA141120.11046947177086978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1070650.096.881821
AGCAGTG1157700.089.471642
GCAGTGG1276100.080.9535453
CAGTGGT1296600.079.719474
GGTATCA1308600.078.9254468
GTGGTAT1315600.078.531316
TGGTATC1317150.078.45477
AGTGGTA1321700.078.213065
GTATCAA1334750.077.211979
GCAGAGT1335150.042.07074716-17
CAACGCA1336700.042.0703912-13
AGAGTAC1336900.041.8077718-19
ATCAACG1363100.041.2995310-11
TACATGG1360050.040.75192622-23
CATGGGG488800.039.77163724-25
AGTACAT1403550.039.71487420-21
CAGAGTA1343150.038.36933518-19
ACGCAGA1469150.038.3449514-15
AACGCAG1348250.038.3340814-15
TCAACGC1358700.038.06205412-13
Produced by FastQC (version 0.11.5)