FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15582929
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC589770.37847185211458No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG204940.13151571184082275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181250.11631317835048853No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA171680.11017184253358274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1285450.096.989661
AGCAGTG1392900.089.354332
GCAGTGG1550700.080.0539863
CAGTGGT1579150.078.698884
GGTATCA1591700.078.0773858
GTGGTAT1593800.077.974516
AGTGGTA1601350.077.662065
TGGTATC1604200.077.526597
GTATCAA1629950.076.232639
GCAGAGT1623100.041.83838716-17
CAACGCA1629250.041.81168712-13
AGAGTAC1626800.041.59634418-19
ATCAACG1657200.041.13473510-11
TACATGG1657300.040.42142522-23
CATGGGG594250.039.43327324-25
AGTACAT1712450.039.34432220-21
ACGCAGA1793300.038.0552814-15
AACGCAG1637950.038.0253714-15
CAGAGTA1633950.037.9713518-19
TCAACGC1651900.037.6865812-13
Produced by FastQC (version 0.11.5)