FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_1-SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_1-SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22464904
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGCTATTTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAAC832862237.073926512216566No Hit
TGCCGCTATTTCTCTGGGTACCTCTGCATGATTCTCGCTATTATTCCAAC621822927.679748820649312No Hit
TGCCGCTATTTCTCTGGGTACCGTAATCGTGTTCTCGCTATTATTCCAAC292851613.035960447460626No Hit
TGCCGCTATTTCTCTGGGTACCGCGTATAGCTTCTCGCTATTATTCCAAC241448610.747813567331514No Hit
TGCCGCTATTTCTCTGGGTACCGAGCGATCTTTCTCGCTATTATTCCAAC1809900.8056566811948095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1233730.5491810692803317No Hit
TGCCGCTATTTCTCTGGGTACCAACACTTACTTCTCGCTATTATTCCAAC737830.32843674738160467No Hit
TGCCGCTATTTCTCTGGGTACCCAATGTTGTTTCTCGCTATTATTCCAAC488780.21757493377225204No Hit
TGCCGCTATTTCTCTGGGTACCTATTTGTGTTTCTCGCTATTATTCCAAC419800.18686926060311673No Hit
TGCCGCTATTCTCTGGGTACCCTACGTACGTTCTCGCTATTATTCCAACC233210.10381081530550942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTT154.590449E-4104.161631
TGCCTAT3000.0104.161611
TGCCGCT21721300.0104.1061
TCCGCGA306.553819E-9104.047722
TCCGCAA500.0104.047722
TCCGATA500.0104.047722
GCCTTTC700.0104.0477142
GCCTCTA14400.0104.0477142
ATGTACT154.6123058E-4104.03729104
CCTGGCG154.6123058E-4104.03729104
ACGGTTA1650.0104.037285104
CCACAAG154.6183544E-4104.003013
CCTGCTA154.6183544E-4104.003013
CCTGCAG154.6183544E-4104.003013
CCGGTAG450.0104.0030063
CCGCCAT4700.0104.0030063
CGTCTCT252.6980888E-7103.9898154
ATGGCTC154.622404E-4103.9800958
TCCTCAC201.1139407E-5103.9800958
GCCGCTA21761750.0103.923882
Produced by FastQC (version 0.11.5)