FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17459251
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC637350.3650500241963415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209320.1198905955358566No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG204770.11728452726866691No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA175330.10042240643656478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1370850.096.6611251
AGCAGTG1495300.088.579492
GCAGTGG1672400.079.0552143
CAGTGGT1709150.077.391864
GTGGTAT1728850.076.437046
GGTATCA1733650.076.203988
AGTGGTA1742300.075.94745
TGGTATC1766050.074.899937
GTATCAA1771750.074.480189
GCAGAGT1762900.040.98105216-17
CAACGCA1768500.040.9398412-13
AGAGTAC1785000.040.35296618-19
ATCAACG1813150.039.99337410-11
TACATGG1800300.039.61875522-23
CATGGGG792650.039.0412524-25
AGTACAT1868950.038.38876320-21
CAGAGTA1780100.037.0584518-19
AACGCAG1786200.037.03410714-15
ACGCAGA1967950.036.82501614-15
TCAACGC1804000.036.7075512-13
Produced by FastQC (version 0.11.5)