FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19126169
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC708780.3705812700912556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222160.1161549916243028No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG221950.11604519441399895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1516500.096.982381
AGCAGTG1649500.088.976392
GCAGTGG1839150.079.683063
CAGTGGT1884250.077.862964
GTGGTAT1904350.077.009926
GGTATCA1912250.076.789058
AGTGGTA1917200.076.564365
TGGTATC1942950.075.586237
GTATCAA1953400.075.052049
CAACGCA1949150.041.12214312-13
GCAGAGT1947050.041.07716-17
AGAGTAC1969500.040.4662218-19
ATCAACG2000750.040.1187610-11
TACATGG1985450.039.73524522-23
CATGGGG868400.039.08669324-25
AGTACAT2062400.038.5249520-21
CAGAGTA1963050.037.29382318-19
AACGCAG1970100.037.26358814-15
TCAACGC1991050.036.91182312-13
ACGCAGA2176300.036.85885614-15
Produced by FastQC (version 0.11.5)