FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17228324
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC632590.36718023180896764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204160.11850253106454231No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG201910.11719654215929536No Hit
CCCGATTTGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTA174540.10130991267635783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1358550.096.5625461
AGCAGTG1484600.088.2741552
GCAGTGG1649050.079.36023
CAGTGGT1686000.077.599764
GTGGTAT1708700.076.5617456
GGTATCA1719200.076.11148
AGTGGTA1725400.075.8444445
TGGTATC1746850.074.980867
GTATCAA1753400.074.502979
CAACGCA1747600.041.1418212-13
GCAGAGT1752300.041.00280816-17
AGAGTAC1766100.040.5253318-19
ATCAACG1794850.040.0904410-11
TACATGG1783200.039.74751722-23
CATGGGG779000.039.27546724-25
AGTACAT1850100.038.53365720-21
CAGAGTA1761350.037.12921518-19
AACGCAG1765500.037.08367514-15
ACGCAGA1952850.036.88723814-15
TCAACGC1783200.036.7240112-13
Produced by FastQC (version 0.11.5)