FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n02_CRISPR_18hpf_202008_1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n02_CRISPR_18hpf_202008_1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396963
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC568390.3691572162640126No Hit
CTTTTTTATCATGGGAGCCTACTTCCCGTTTTTCCCGATTTGGCTACATG181870.11812069691925609No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177560.1153214435859851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1216000.096.642121
AGCAGTG1327550.088.403652
GCAGTGG1480650.079.145513
CAGTGGT1514950.077.328034
GGTATCA1540850.075.95318
GTGGTAT1542300.075.932756
AGTGGTA1550200.075.631845
TGGTATC1568100.074.745227
GTATCAA1576700.074.0909359
GCAGAGT1567650.040.85652516-17
CAACGCA1572350.040.78333312-13
AGAGTAC1584250.040.2662318-19
ATCAACG1609700.039.86758810-11
TACATGG1594900.039.5882422-23
CATGGGG705050.038.64870524-25
AGTACAT1658350.038.32123620-21
CAGAGTA1580900.036.9904418-19
AACGCAG1584600.036.9724314-15
ACGCAGA1752500.036.63725314-15
TCAACGC1601000.036.6177612-13
Produced by FastQC (version 0.11.5)