FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9679904
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG134370.13881336013249718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG35100.019.5769622
TGTCGAG36350.019.5082686
TAAGCAC57250.019.4207951
GGTGCAA36450.019.205117
GGGGTGA16800.019.1743985
ACGGTGC40650.019.0944675
ATAATGC43700.018.9861079
GGGCTCC30700.018.9802064
GATATCC34900.018.8696924
GGGAGGT32900.018.7469395
CCGGGAC20350.018.738539
GAAAGGA58500.018.718325
TACGCCT38650.018.717542
CCCATGG25500.018.5396122
AAAGGGC45350.018.52711
GTCGAGA37350.018.4775337
CGTGGCT29400.018.465039
AGGCTGC49800.018.3069731
GGCTGCA36300.018.2937492
GAGCGAA23850.018.2926677
Produced by FastQC (version 0.11.5)