FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_2-SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_2-SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18582439
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAGGA416150.021.516135
GCGAGAA298150.021.4427621
GGTGCAA311350.021.3960137
AATACGG312850.021.3931732
CGAGAAA297550.021.3763142
AGAAAGG299500.021.2584384
AGGCTGC345250.021.2562031
AAGAGCG165700.021.2442535
GAGCGAA166300.021.2227487
TTACGCC338650.021.208911
ACGGTGC334950.021.1997835
CCCGGAA176400.021.197561
TACGGTG314100.021.191684
AGAGCGA167150.021.1784636
TGTCGAG262050.021.1765176
ACCTGAC216950.021.1616542
AGAAGAG167600.021.12163
CGGTGCA315600.021.1153666
AACCTGA222300.021.0890521
ACGCCTC292100.021.0812283
Produced by FastQC (version 0.11.5)