FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14618037
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTC119000.020.9075342
ATAATGC219100.020.7669359
TGGGAGG132200.020.7588734
CATAATG220450.020.6647078
TTGGGAG147000.020.6375543
GGGAGGT132750.020.5402415
GTCCCAT232400.020.4922644
GGAGGTC120400.020.362336
GAGGTCG97900.020.332684
CGGGTCA123200.020.2568133
ATGAAAC82400.020.24825510
TAAGCAC95250.020.2108211
CGTCCCA119450.020.2018573
TCGTCCC119850.020.0890062
TGAGACT93850.020.0431961
AAGAGCC70550.019.9329995
GGGTCAT124700.019.871964
TATGAAA83300.019.8578439
TGCGGGT126200.019.8330861
CTCGAGG104300.019.8184851
Produced by FastQC (version 0.11.5)