FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18054895
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGC270700.020.8630819
GCGGGTC145600.020.7918192
TTGGGAG178100.020.6732353
TGGGAGG159250.020.6534064
CATAATG275400.020.5709278
GGGAGGT159200.020.5147425
GTCCCAT281000.020.4922494
CGTCCCA145400.020.441213
GAGGTCG123350.020.3841344
TCGTCCC145700.020.3621482
GGAGGTC145950.020.3038046
AAGAGCC89450.020.1501735
CTCGAGG127450.020.1228771
TAAGCAC118350.020.0704521
TGAGACT116750.019.9872471
CGGGTCA151850.019.9715063
ATGAAAC100050.019.92998510
GGGTCAT151950.019.8134734
GAGGTCA150550.019.7903987
ATCGTCC167800.019.718371
Produced by FastQC (version 0.11.5)