FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12774570
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTC104200.020.8579352
ATAATGC195100.020.8181579
TGGGAGG115550.020.7898144
TTGGGAG129300.020.6215693
CATAATG198850.020.480878
GTCCCAT198050.020.464154
GGGAGGT117350.020.4615485
CGTCCCA103350.020.2732473
GGAGGTC105400.020.2652426
GAGGTCG84200.020.218324
ATGAAAC71100.020.15557110
CGGGTCA108400.020.1206133
TCGTCCC104300.020.0782742
CCAAGAC57300.020.0577377
CTCGAGG86550.020.0430971
GGGTCAT108150.020.0348474
TGAGACT78400.020.0347331
TAAGCAC81200.020.0216661
TATGAAA70650.020.003749
TGCGGGT109700.019.9674281
Produced by FastQC (version 0.11.5)