FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15582929
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT137950.020.9470845
ATAATGC237150.020.8603279
GCGGGTC125250.020.8402462
TGGGAGG139700.020.8185924
CATAATG240250.020.6277838
TTGGGAG156300.020.5648023
GTCCCAT244950.020.4132184
GAGGTCG102000.020.4005534
GGAGGTC127450.020.315746
CTCGAGG107050.020.1325741
TCGTCCC126250.020.117332
CGTCCCA126450.020.1022723
TAAGCAC98150.020.0965271
TGCGGGT130750.020.0695611
GAGGTCA130300.019.9792257
CGGGTCA131550.019.9670333
ATGAAAC86500.019.96219610
CCAAGAC75100.019.7998337
AAGAGCC77950.019.7916725
TATGAAA86050.019.78549
Produced by FastQC (version 0.11.5)