FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_1-SBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_1-SBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22464904
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCAC533200.021.351671
AAAGGCG239050.021.203316
AAAGGAG179000.021.1297919
GGCGATA240650.021.1277929
AGTGAGA303950.021.0264427
TGGAACC205900.021.0239759
AAGGAAA245100.021.0134777
GCGATAC241900.020.99588410
AAGGCGA241750.020.9771127
AGGCGAT242500.020.966668
CGTACAA355200.020.96517
GGAACCA207150.020.95551310
GTGGAAC208000.020.9492368
CGTGGAA207400.020.9037847
CCGTGGA207500.020.8952036
AGATAGA310300.020.8918651
AGCACGT396350.020.8622783
GTGGGTG194100.020.8069764
TAGAGTG309050.020.7555274
GATAGAG307700.020.7545282
Produced by FastQC (version 0.11.5)