FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17459251
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCC243700.020.3944681
CAAACGG135350.020.33876210
CGAGGAT123050.020.25350810
ACGAGGA123000.020.2527989
CGCGATG92650.020.22768210
TGCGGAG82900.020.2161623
GAGGTGA83550.020.2037035
CGCGTGA114950.020.1774251
GAGGAGG83950.020.1737292
GCGGAGA57900.020.1265564
AATCAGC132250.020.077696
CCGCGAT93650.020.0234349
AGGAGAC91400.019.9854555
TCGTAGC205050.019.9850398
AAAGGGC118650.019.9564291
CTCCGCG93650.019.9412277
TCGGATG95100.019.9376072
GGAGGTG85300.019.8666114
ATCGGAT165500.019.861041
GAGACGC54800.019.8288887
Produced by FastQC (version 0.11.5)