FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19126169
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGAT139600.020.49941410
ACGAGGA139700.020.476879
CGCGATG96200.020.43003510
ACGTTCC273800.020.407481
CAAACGG146450.020.33662610
CGCGTGA120100.020.3291761
AAAGGGC129100.020.284741
GAGGTGA92250.020.1833485
AGGAGAC104200.020.1708815
TGCGGAG93550.020.067713
AATCAGC143700.020.0480486
TCGGATG103900.020.0073872
GCGGAGA63950.019.9607944
ATCGGAT179050.019.9560891
GCAATCA144100.019.9467353
CTCCGCG98650.019.9449467
GAGGAGG93700.019.9421562
GGAGGTG93750.019.9191554
GAGACGC64500.019.9160277
TCGTAGC230550.019.9068668
Produced by FastQC (version 0.11.5)