FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17228324
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA110000.020.6758481
ACGAGGA126400.020.4994819
ACGTTCC240000.020.4390031
GAGGTGA80400.020.3529225
CGCGATG87650.020.35230410
CGAGGAT127500.020.3053710
GAGGAGG80500.020.3009472
CAAACGG132750.020.28938710
AAAGGGC117950.020.2529141
CCGCGAT88300.020.2273949
GCGGAGA56950.020.1733784
AGGAGAC93750.020.1544485
GGAGGTG81700.020.1233564
ATCGGAT161650.020.0555861
TGCGGAG82650.020.02523
AATCAGC126850.020.022456
TCGGATG92800.020.0056572
CGGATGT93150.019.9298523
TCGTAGC201950.019.8612488
TTCCTCG210600.019.845794
Produced by FastQC (version 0.11.5)