FastQCFastQC Report
Thu 13 Aug 2020
HFW2LAFX2_n01_CRISPR_18hpf_202008_1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFW2LAFX2_n01_CRISPR_18hpf_202008_1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396963
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA98300.020.7945821
CAAACGG120650.020.49238810
GAGGAGG72500.020.4456082
ACGTTCC217150.020.4437331
GAGGAGA55650.020.2876384
ACGAGGA108800.020.2476989
AGGAGAC79050.020.1565385
CCGCGAT81750.020.1533689
CGCGATG81550.020.1353610
TCGGATG84100.020.1247632
AAAGGGC103500.020.1007981
CGAGGAT109650.020.08070810
GCGGAGA51800.020.0323184
TGCGGAG73100.020.0210293
CGGATGT84550.020.0037943
TTCCTCG190400.019.956254
TCGTAGC182200.019.9501728
ATCGGAT146150.019.9364091
GAGGTGA74100.019.9289135
GGAGGTG75100.019.8833334
Produced by FastQC (version 0.11.5)