FastQCFastQC Report
Thu 6 Apr 2023
HFVVWAFX5_n01_7799_midlog_Rep2_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVVWAFX5_n01_7799_midlog_Rep2_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6167768
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC592060.9599258597275383TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC181340.2940123558473665TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC66920.1084995414872933TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC63150.1023871196192853TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC63110.10232226633686611TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)