FastQCFastQC Report
Thu 20 Aug 2020
HFVTVAFX2_n02_vk7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFVTVAFX2_n02_vk7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9199330
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266510.28970588075435927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC29100.027.30284330
GCGAGCG15450.025.8227111
GGCGAGC16300.024.04449810
CGGCGGC32700.023.86875733
TCGGCGG34550.022.79329732
GTCGGCG35800.022.19297231
CGAGCGT17800.022.02031112
CGCGAAG10200.021.6411741
CGAGCAG14750.021.3791731
GAATGTC39000.021.1823627
CGGCAAG16750.021.1274451
CGCGCAG3900.020.6634881
CGCGAAA11200.020.3346041
GGATCCG18650.020.2657837
GTGGGAC40000.020.04021819
GGCGGCA41100.019.1605834
GAAGCGT46650.019.13536569
CCGCAAG12650.019.1116851
TGCGTCG6300.018.9094451
GCCGTGT14450.018.65396170
Produced by FastQC (version 0.11.5)